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Fisher Scientific superscript iv first- strand synthesis system
Superscript Iv First Strand Synthesis System, supplied by Fisher Scientific, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/superscript+iv+first-strand+synthesis+system/superscript+iv+synthesis+system/pm40641092-61-17-24
Average 90 stars, based on 1 article reviews
superscript iv first- strand synthesis system - by Bioz Stars, 2026-09
90/100 stars

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Recombinant:

Article Title: Advanced Glycation End Products and Receptor (RAGE) Promote Wound Healing of Human Corneal Epithelial Cells
Article Snippet: HMGB1 (SRP6265, a mixture of different forms) was obtained from Sigma-Aldrich, HMGB1 in reduced form (HM-131) was obtained from HMGBiotech (Milano, Italia), and AGEs (ab51995, obtained by reaction between BSA and glycolaldehyde) were purchased from Abcam (Paris, France). .. SuperScript IV First-Strand Synthesis System, Taq DNA Polymerase recombinant (10342020), Pierce BCA Protein Assay Kit (23225), Lipofectamine 3000 Transfection Reagent (L3000008), and Lipofectamine RNAiMAX Transfection Reagent (13778150) were purchased from Fisher Scientific. .. LightCycler 480 SYBR Green I Master (04887352001) was provided by Roche (Meylan, France).

Bicinchoninic Acid Protein Assay:

Article Title: Advanced Glycation End Products and Receptor (RAGE) Promote Wound Healing of Human Corneal Epithelial Cells
Article Snippet: HMGB1 (SRP6265, a mixture of different forms) was obtained from Sigma-Aldrich, HMGB1 in reduced form (HM-131) was obtained from HMGBiotech (Milano, Italia), and AGEs (ab51995, obtained by reaction between BSA and glycolaldehyde) were purchased from Abcam (Paris, France). .. SuperScript IV First-Strand Synthesis System, Taq DNA Polymerase recombinant (10342020), Pierce BCA Protein Assay Kit (23225), Lipofectamine 3000 Transfection Reagent (L3000008), and Lipofectamine RNAiMAX Transfection Reagent (13778150) were purchased from Fisher Scientific. .. LightCycler 480 SYBR Green I Master (04887352001) was provided by Roche (Meylan, France).

Transfection:

Article Title: Advanced Glycation End Products and Receptor (RAGE) Promote Wound Healing of Human Corneal Epithelial Cells
Article Snippet: HMGB1 (SRP6265, a mixture of different forms) was obtained from Sigma-Aldrich, HMGB1 in reduced form (HM-131) was obtained from HMGBiotech (Milano, Italia), and AGEs (ab51995, obtained by reaction between BSA and glycolaldehyde) were purchased from Abcam (Paris, France). .. SuperScript IV First-Strand Synthesis System, Taq DNA Polymerase recombinant (10342020), Pierce BCA Protein Assay Kit (23225), Lipofectamine 3000 Transfection Reagent (L3000008), and Lipofectamine RNAiMAX Transfection Reagent (13778150) were purchased from Fisher Scientific. .. LightCycler 480 SYBR Green I Master (04887352001) was provided by Roche (Meylan, France).

Control:

Article Title: Role of clonal inflammatory microglia in histiocytosis-associated neurodegeneration.
Article Snippet: RNA was isolated from cell pellets from dissected brain regions using RNA using the Qiagen all-prep DNA/RNA mini kit (80204) according to the manufacturer’s instructions. .. RNAs were used for ribogreen quantification and quality control on Agilent BioAnalyzer. cDNA was prepared using SuperScript IV First-Strand Synthesis System (FisherScientific) following manufacturers instructions. .. Resulting cDNAwas used for q-PCR using TaqmanGene Expression Assays using TaqMan Fast AdvancedMaster Mix (Applied Biosystems).

Reverse Transcription:

Article Title: Vitamin B3, nicotinamide, enhances mitochondrial metabolism to promote differentiation of the retinal pigment epithelium
Article Snippet: Total RNA was harvested from ARPE-19 cells cultured on plastic surfaces for 2 weeks and hfRPE cells cultured for 4 weeks in MEM or MEM-Nic, using the PureLink RNA Mini Kit (Fisher Scientific). .. Reverse transcription was performed with the SuperScript IV First-Strand Synthesis System (Fisher Scientific) to generate complementary DNA from approximately 3 to 5 μg of total RNA. .. The complementary DNA was amplified using KAPA SYBR Green mastermix (KAPA Biosystems) on the Roche LightCycler 480 Instrument II.

Article Title: Stromal androgen signaling acts as tumor niches to drive prostatic basal epithelial progenitor-initiated oncogenesis
Article Snippet: .. mRNA was extracted and purified from sorted cells using TRIZOL (Zymo Research) and reverse-transcribed using SuperScript IV First-Strand Synthesis System (Fisher Scientific) according to the manufacturer’s protocol. qRT-PCR reactions were performed in triplicate using SYBR Green PCR master mix (Applied Biosystems) with specific primers (Supplementary Table ) on the 7500 Real-time PCR system (Thermo Fisher Scientific). ..

Purification:

Article Title: Stromal androgen signaling acts as tumor niches to drive prostatic basal epithelial progenitor-initiated oncogenesis
Article Snippet: .. mRNA was extracted and purified from sorted cells using TRIZOL (Zymo Research) and reverse-transcribed using SuperScript IV First-Strand Synthesis System (Fisher Scientific) according to the manufacturer’s protocol. qRT-PCR reactions were performed in triplicate using SYBR Green PCR master mix (Applied Biosystems) with specific primers (Supplementary Table ) on the 7500 Real-time PCR system (Thermo Fisher Scientific). ..

Quantitative RT-PCR:

Article Title: Stromal androgen signaling acts as tumor niches to drive prostatic basal epithelial progenitor-initiated oncogenesis
Article Snippet: .. mRNA was extracted and purified from sorted cells using TRIZOL (Zymo Research) and reverse-transcribed using SuperScript IV First-Strand Synthesis System (Fisher Scientific) according to the manufacturer’s protocol. qRT-PCR reactions were performed in triplicate using SYBR Green PCR master mix (Applied Biosystems) with specific primers (Supplementary Table ) on the 7500 Real-time PCR system (Thermo Fisher Scientific). ..

SYBR Green Assay:

Article Title: Stromal androgen signaling acts as tumor niches to drive prostatic basal epithelial progenitor-initiated oncogenesis
Article Snippet: .. mRNA was extracted and purified from sorted cells using TRIZOL (Zymo Research) and reverse-transcribed using SuperScript IV First-Strand Synthesis System (Fisher Scientific) according to the manufacturer’s protocol. qRT-PCR reactions were performed in triplicate using SYBR Green PCR master mix (Applied Biosystems) with specific primers (Supplementary Table ) on the 7500 Real-time PCR system (Thermo Fisher Scientific). ..

Polymerase Chain Reaction:

Article Title: Stromal androgen signaling acts as tumor niches to drive prostatic basal epithelial progenitor-initiated oncogenesis
Article Snippet: .. mRNA was extracted and purified from sorted cells using TRIZOL (Zymo Research) and reverse-transcribed using SuperScript IV First-Strand Synthesis System (Fisher Scientific) according to the manufacturer’s protocol. qRT-PCR reactions were performed in triplicate using SYBR Green PCR master mix (Applied Biosystems) with specific primers (Supplementary Table ) on the 7500 Real-time PCR system (Thermo Fisher Scientific). ..

Real-time Polymerase Chain Reaction:

Article Title: Stromal androgen signaling acts as tumor niches to drive prostatic basal epithelial progenitor-initiated oncogenesis
Article Snippet: .. mRNA was extracted and purified from sorted cells using TRIZOL (Zymo Research) and reverse-transcribed using SuperScript IV First-Strand Synthesis System (Fisher Scientific) according to the manufacturer’s protocol. qRT-PCR reactions were performed in triplicate using SYBR Green PCR master mix (Applied Biosystems) with specific primers (Supplementary Table ) on the 7500 Real-time PCR system (Thermo Fisher Scientific). ..



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